Table 2 Results of diagnostic tests and treatment provided to leishmaniasis cases
From: Imported leishmaniasis cases in Cuba (2006–2016): what have we learned
Case number | Occupation | Sample | Direct smear | Culture | Histopathology | PCR-18S | PCR-hsp20 | PCR-hsp70 | Leishmania spp. identified | Treatment regime |
|---|---|---|---|---|---|---|---|---|---|---|
Case-1 | Not known | Biopsy | Positive | Positive | Not done | Positive | Positive | Positive | L.(V.) braziliensis | Glucantime (20 mg/Kg/day) for 20 days* |
Case-2 | Student | Biopsy | Not done | Contaminated | Elements in macrophages compatible with Leishmania amastigotes | Positive | Positive | Positive | L. (L.) mexicana | Amphotericin B 25 mg × 3 days 50 mg × 12 days |
Case-4 | Student | Biopsy | Negative | Positive contaminated | Elements similar to amastigotes in macrophages. No kinetoplast identified | Positive | Positive | Positive weak | No identified | Liposomal Amphotericin B 150 mg days 1–5 followed by 200 mg weekly × 3 weeks |
Case-11 | Physic education trainer | Biopsy embedded paraffin | Not done | Not done | Leishmania amastigotes (kinetoplast) in macrophages | Positive | Positive | Negative | No identified | Liposomal Amphotericin B 150 mg days 1–3 followed by 200 mg biweekly for three weeks |
Case-14 | Art Instructor | Scraping | Positive | Negative | Not done | Positive | Not done | Negative | No identified | Glucantime (20 mg/Kg/day) for 28 days |