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Table 1 Parasitologic factors, quantitative PCR results, and RNA transcript expression in 45 Plasmodium falciparum positive specimens with isolated gametocytemia by expert microscopy

From: Correlating quantitative real-time PCR to rapid diagnostic test and RNA transcript expression in isolated gametocytemia and asexual parasitemia of Plasmodium falciparum malaria

Sample Microscopy Parasitemia HRP-2 T1 Aldolase T2 Plasmodium Genus Mean CT StDev P. falciparum Mean CT StDev Ave 18S rRNA gene copy#/rxn eba-175 myosin
1 Gametocytes <0.1 + - 39.8 0.1 - - 4 + +
2 Gametocytes <0.1 + - - - 39.8 0.8 6 + -
3 Gametocytes <0.1 + - 38.5 0.6 37.7 0.8 30 + +
4 Gametocytes <0.1 - - 38.3 0.1 36.9 0.5 43 ND ND
5 Gametocytes <0.1 - - 37.5 0.1 37.5 0.9 69 ND ND
6 Gametocytes <0.1 + - 35.5 0.4 34.8 1.0 287 ND ND
7 Gametocytes <0.1 + - 35.1 0.2 34.6 1.1 331 ND ND
8 Gametocytes <0.1 + - 35.5 0.6 33.6 1.4 506 ND ND
9 Gametocytes <0.1 + - 33.4 0.1 33.8 1.7 672 ND ND
10 Gametocytes <0.1 + - 34.2 0.2 33.3 1.4 687 ND ND
11 Gametocytes <0.1 + - 33.2 0.1 33.4 1.0 803 ND ND
12 Gametocytes <0.1 + - 34.1 0.4 32.4 1.1 814 ND ND
13 Gametocytes <0.1 + - 33.0 0.1 32.7 0.9 1046 ND ND
14 Gametocytes <0.1 + + 33.2 0.3 32.4 0.8 1195 + +
15 Gametocytes <0.1 + - 33.6 0.6 31.3 0.3 1214 - +
16 Gametocytes <0.1 + - 32.5 0.4 31.7 0.5 1251 + +
17 Gametocytes <0.1 + - 32.5 0.6 32.2 0.8 1422 + +
18 Gametocytes <0.1 + - 33.5 0.4 31.1 0.8 1525 ND ND
19 Gametocytes <0.1 + - 31.8 0.4 32.1 1.0 1754 ND ND
20 Gametocytes <0.1 + - 31.6 0.3 32.1 1.2 1801 ND ND
21 Gametocytes <0.1 + - 32.3 0.6 31.7 1.1 1854 ND ND
22 Gametocytes <0.1 + - 31.5 0.3 31.3 0.0 1905 + +
23 Gametocytes <0.1 - - 31.5 0.3 30.5 0.4 2530 - +
24 Gametocytes <0.1 + - 32.2 0.8 30.9 0.8 2573 - +
25 Gametocytes <0.1 + - 32.0 0.5 30.4 0.9 3267 - +
26 Gametocytes <0.1 - - 30.7 0.3 31.0 1.2 3300 - +
27 Gametocytes <0.1 + - 31.3 0.1 30.5 0.9 3422 ND ND
28 Gametocytes <0.1 + - 30.2 0.3 29.9 0.2 4374 + +
29 Gametocytes <0.1 + - 29.9 0.4 30.0 0.9 5640 ND ND
30 Gametocytes <0.1 + - 30.2 0.1 29.7 0.7 5928 - +
31 Gametocytes <0.1 + + 30.7 0.8 28.8 0.5 5967 + +
32 Gametocytes <0.1 + + 30.0 0.1 29.3 0.8 7246 ND ND
33 Gametocytes <0.1 + + 29.8 0.2 29.2 0.8 7694 - +
34 Gametocytes <0.1 + + 29.6 0.2 28.9 0.9 8996 - +
35 Gametocytes <0.1 + - 29.3 0.1 28.9 1.1 9686 + +
36 Gametocytes <0.1 + + 28.6 0.4 29.1 1.0 10539 ND ND
37 Gametocytes <0.1 - + 28.8 0.2 28.5 0.5 11110 ND ND
38 Gametocytes <0.1 + + 28.4 0.7 27.5 0.5 14285 + +
39 Gametocytes <0.1 + + 28.2 0.4 27.5 0.2 18236 + +
40 Gametocytes <0.1 + + 27.6 0.3 27.9 1.1 20566 - +
41 Gametocytes <0.1 + + 26.9 0.3 26.7 0.2 34485 - +
42 Gametocytes <0.1 + - 26.7 0.2 26.4 0.6 42605 ND ND
43 Gametocytes <0.1 + + 26.3 0.7 26.2 0.3 50031 - +
44 Gametocytes <0.1 + + 26.5 0.1 26.0 0.8 51397 - +
45 Gametocytes <0.1 + + 26.2 0.3 25.5 0.1 65208 - +
  1. ND not determined