Table 1 Parasitologic factors, quantitative PCR results, and RNA transcript expression in 45 Plasmodium falciparum positive specimens with isolated gametocytemia by expert microscopy
Sample | Microscopy | Parasitemia | HRP-2 T1 | Aldolase T2 | Plasmodium Genus Mean CT | StDev | P. falciparum Mean CT | StDev | Ave 18S rRNA gene copy#/rxn | eba-175 | myosin |
|---|---|---|---|---|---|---|---|---|---|---|---|
1 | Gametocytes | <0.1 | + | - | 39.8 | 0.1 | - | - | 4 | + | + |
2 | Gametocytes | <0.1 | + | - | - | - | 39.8 | 0.8 | 6 | + | - |
3 | Gametocytes | <0.1 | + | - | 38.5 | 0.6 | 37.7 | 0.8 | 30 | + | + |
4 | Gametocytes | <0.1 | - | - | 38.3 | 0.1 | 36.9 | 0.5 | 43 | ND | ND |
5 | Gametocytes | <0.1 | - | - | 37.5 | 0.1 | 37.5 | 0.9 | 69 | ND | ND |
6 | Gametocytes | <0.1 | + | - | 35.5 | 0.4 | 34.8 | 1.0 | 287 | ND | ND |
7 | Gametocytes | <0.1 | + | - | 35.1 | 0.2 | 34.6 | 1.1 | 331 | ND | ND |
8 | Gametocytes | <0.1 | + | - | 35.5 | 0.6 | 33.6 | 1.4 | 506 | ND | ND |
9 | Gametocytes | <0.1 | + | - | 33.4 | 0.1 | 33.8 | 1.7 | 672 | ND | ND |
10 | Gametocytes | <0.1 | + | - | 34.2 | 0.2 | 33.3 | 1.4 | 687 | ND | ND |
11 | Gametocytes | <0.1 | + | - | 33.2 | 0.1 | 33.4 | 1.0 | 803 | ND | ND |
12 | Gametocytes | <0.1 | + | - | 34.1 | 0.4 | 32.4 | 1.1 | 814 | ND | ND |
13 | Gametocytes | <0.1 | + | - | 33.0 | 0.1 | 32.7 | 0.9 | 1046 | ND | ND |
14 | Gametocytes | <0.1 | + | + | 33.2 | 0.3 | 32.4 | 0.8 | 1195 | + | + |
15 | Gametocytes | <0.1 | + | - | 33.6 | 0.6 | 31.3 | 0.3 | 1214 | - | + |
16 | Gametocytes | <0.1 | + | - | 32.5 | 0.4 | 31.7 | 0.5 | 1251 | + | + |
17 | Gametocytes | <0.1 | + | - | 32.5 | 0.6 | 32.2 | 0.8 | 1422 | + | + |
18 | Gametocytes | <0.1 | + | - | 33.5 | 0.4 | 31.1 | 0.8 | 1525 | ND | ND |
19 | Gametocytes | <0.1 | + | - | 31.8 | 0.4 | 32.1 | 1.0 | 1754 | ND | ND |
20 | Gametocytes | <0.1 | + | - | 31.6 | 0.3 | 32.1 | 1.2 | 1801 | ND | ND |
21 | Gametocytes | <0.1 | + | - | 32.3 | 0.6 | 31.7 | 1.1 | 1854 | ND | ND |
22 | Gametocytes | <0.1 | + | - | 31.5 | 0.3 | 31.3 | 0.0 | 1905 | + | + |
23 | Gametocytes | <0.1 | - | - | 31.5 | 0.3 | 30.5 | 0.4 | 2530 | - | + |
24 | Gametocytes | <0.1 | + | - | 32.2 | 0.8 | 30.9 | 0.8 | 2573 | - | + |
25 | Gametocytes | <0.1 | + | - | 32.0 | 0.5 | 30.4 | 0.9 | 3267 | - | + |
26 | Gametocytes | <0.1 | - | - | 30.7 | 0.3 | 31.0 | 1.2 | 3300 | - | + |
27 | Gametocytes | <0.1 | + | - | 31.3 | 0.1 | 30.5 | 0.9 | 3422 | ND | ND |
28 | Gametocytes | <0.1 | + | - | 30.2 | 0.3 | 29.9 | 0.2 | 4374 | + | + |
29 | Gametocytes | <0.1 | + | - | 29.9 | 0.4 | 30.0 | 0.9 | 5640 | ND | ND |
30 | Gametocytes | <0.1 | + | - | 30.2 | 0.1 | 29.7 | 0.7 | 5928 | - | + |
31 | Gametocytes | <0.1 | + | + | 30.7 | 0.8 | 28.8 | 0.5 | 5967 | + | + |
32 | Gametocytes | <0.1 | + | + | 30.0 | 0.1 | 29.3 | 0.8 | 7246 | ND | ND |
33 | Gametocytes | <0.1 | + | + | 29.8 | 0.2 | 29.2 | 0.8 | 7694 | - | + |
34 | Gametocytes | <0.1 | + | + | 29.6 | 0.2 | 28.9 | 0.9 | 8996 | - | + |
35 | Gametocytes | <0.1 | + | - | 29.3 | 0.1 | 28.9 | 1.1 | 9686 | + | + |
36 | Gametocytes | <0.1 | + | + | 28.6 | 0.4 | 29.1 | 1.0 | 10539 | ND | ND |
37 | Gametocytes | <0.1 | - | + | 28.8 | 0.2 | 28.5 | 0.5 | 11110 | ND | ND |
38 | Gametocytes | <0.1 | + | + | 28.4 | 0.7 | 27.5 | 0.5 | 14285 | + | + |
39 | Gametocytes | <0.1 | + | + | 28.2 | 0.4 | 27.5 | 0.2 | 18236 | + | + |
40 | Gametocytes | <0.1 | + | + | 27.6 | 0.3 | 27.9 | 1.1 | 20566 | - | + |
41 | Gametocytes | <0.1 | + | + | 26.9 | 0.3 | 26.7 | 0.2 | 34485 | - | + |
42 | Gametocytes | <0.1 | + | - | 26.7 | 0.2 | 26.4 | 0.6 | 42605 | ND | ND |
43 | Gametocytes | <0.1 | + | + | 26.3 | 0.7 | 26.2 | 0.3 | 50031 | - | + |
44 | Gametocytes | <0.1 | + | + | 26.5 | 0.1 | 26.0 | 0.8 | 51397 | - | + |
45 | Gametocytes | <0.1 | + | + | 26.2 | 0.3 | 25.5 | 0.1 | 65208 | - | + |