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Table 2 Samples and diagnostic results of patients with cutaneous leishmaniasis. IPK, Havana, Cuba, 2017

From: Imported cases of cutaneous leishmaniasis in Cuba, 2017: role of human movement

Case Samples Microscopy Histology PCR
18S
PCR
F
RFLP
F
PCRN RFLPN Final Diagnosis
17–01 Lancet scrapings and punch biopsy + + + + L.bra + L.bra L. braziliensis
17–02 Lancet scrapings and punch biopsy + + + + L. pan + L. pan / L. guy L. panamensis
17–03 Lancet scrapings and punch biopsy + + + (w) L. pan / L. guya + L. pan / L. guy L.pan/L.guy
17–04 Lancet scrapings + + + L. pan + L. pan / L.guy L. panamensis
17–05 Lancet scraping + ND + ND + L. mex complex L. mexicana complex
  1. +: Positive result to diagnostic of Leishmania spp
  2. -: Negative result to diagnostic of Leishmania spp
  3. ND Not done
  4. L. bra: Leishmania braziliensis
  5. L. pan: Leishmania panamensis
  6. L. guy: Leishmania guyanensis
  7. L. mex: Leishmania mexicana
  8. Positive (w): A weak amplicon was obtained
  9. a The pattern obtained corresponded to bands characterizing L. panamensis (787 bp, 429 bp) and L. guyanensis (544 bp)