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Table 2 Samples and diagnostic results of patients with cutaneous leishmaniasis. IPK, Havana, Cuba, 2017

From: Imported cases of cutaneous leishmaniasis in Cuba, 2017: role of human movement

Case

Samples

Microscopy

Histology

PCR

18S

PCR

F

RFLP

F

PCRN

RFLPN

Final Diagnosis

17–01

Lancet scrapings and punch biopsy

+

+

+

+

L.bra

+

L.bra

L. braziliensis

17–02

Lancet scrapings and punch biopsy

+

+

+

+

L. pan

+

L. pan / L. guy

L. panamensis

17–03

Lancet scrapings and punch biopsy

+

+

+ (w)

L. pan / L. guya

+

L. pan / L. guy

L.pan/L.guy

17–04

Lancet scrapings

+

+

+

L. pan

+

L. pan / L.guy

L. panamensis

17–05

Lancet scraping

+

ND

+

ND

+

L. mex complex

L. mexicana complex

  1. +: Positive result to diagnostic of Leishmania spp
  2. -: Negative result to diagnostic of Leishmania spp
  3. ND Not done
  4. L. bra: Leishmania braziliensis
  5. L. pan: Leishmania panamensis
  6. L. guy: Leishmania guyanensis
  7. L. mex: Leishmania mexicana
  8. Positive (w): A weak amplicon was obtained
  9. a The pattern obtained corresponded to bands characterizing L. panamensis (787 bp, 429 bp) and L. guyanensis (544 bp)