Molecular characterization of enterotoxigenic Escherichia coli toxins and colonization factors in children under five years with acute diarrhea attending Kisii Teaching and Referral Hospital, Kenya

Table 1 Primers used to detect ETEC enterotoxins and colonization factors [21]

ETEC toxin/colonization factor	Target gene	Primer name	Sequence (5′ to 3′)	Amplicon size (bp)
LT	eltB1	LThF1	CATAATGAGTACTTCGATAGAGGAAC	402
LT	eltB1	LThR1	GAAACCTGCTAATCTGTAACCATCC	402
STp	estA1	STpF1	ATGAAAAAGCTAATGTTGGCA	239
STp	estA1	STpR1	TTAATAACATCCAGCACAGGCA	239
STh	estA2–4	estA2–4F	AATTGCTACTATTCATGCTTTCAGGAC	133
STh	estA2–4	estA2–4R	TCTTTTTCACCTTTCGCTCAGG	133
CFA/I	cfaB	mpcr gp1F	TGAGTGCTTCWGCAGTAGAGA	204
CFA/I	cfaB	CFA1R	CAGCAAGTTTAACAATTACTTTTTTAGT	204
CS4	csaB	mpcr gp1F	TGAGTGCTTCWGCAGTAGAGA	300
CS4	csaB	CS4R	AAGTCACATCTGCGGTTGATAGAG	300
CS14	csuA1	mpcr gp1F	TGAGTGCTTCWGCAGTAGAGA	357
CS14	csuA1	CS14R	TACTATTCGAAACACCTGCCG	357
CS6	cssB	CssBCS6F	GGAGTGGTAAATGCAGGAAACT	416
CS6	cssB	CssbCS6R	GTA CCA GAC GAA TAT CCG CTA TTA	416
CS3	cstA	CS3F1	GGTCTTTCACTGTCAGCTATGAGTT	136
CS3	cstA	CS3R1	TAATGTTAAATTATCCTGAGGAGCC	136
CS5	csfA	CS5F1	GCGTGACACGTCAGCTAATATAAAC	235
CS5	csfA	CS5R	GGCATTCATATCAATAGAAATATGAGAC	235
CS7	csvA	CS7F	TGCTCCCGTTACTAAAAATACG	418
CS7	csvA	CS7R	GGCATTCATATCAATAGAAATATGAGAC	418
CS2	cotA	CS2F	TCTGCTCGTATCAATACCCAAGTT	140
CS2	cotA	CS2R	GTGCCAGCGAATGAAACCTCTAAA	140
CS17/19	csbA and csdA	mpcr GP3	ACTCTRTCGCATTAACCTATTCT	169
CS17/19	csbA and csdA	CS17_19R	GTCACTTTCATCGGAATTTGCGAG	169
CS21	IngA	CS21F	TATGAGCCTKCTGGAAGTYATCAT	292
CS21	IngA	CS21R	GTTATTACGCACTTCGTCTGGT	292
CS1/PCF071	csoA	mpcr gp3	ACTCTRTCGCATTAACCTATTCT	334
CS1/PCF071	csoA	CS1_PCFO71R	CCCTGATATTGACCAGCTGTTAGT	334

The ETEC CFs screening was performed in three separate PCR master mixes: master mix I for detection of CFA/I, CS4, CS6 and CS14 genes, master mix II for detection of CS3, CS5 and CS7 genes and master mix III for detection of CS2, CS17/19, CS21 and CS1/PCF071 genes

ISSN: 2055-0936