Panspecies molecular assays detect viral pathogens missed by real-time PCR/reverse-transcriptase PCR among pneumonia patients, Sarawak, Malaysia

Fieldhouse, Jane K.; Bailey, Emily S.; Toh, Teck-Hock; Hii, King-Ching; Mallinson, Kerry A.; Ting, Jakie; Lednicky, John A.; Berita, Antoinette; Nguyen, Tham Thi; Galan, Diego; Than, Son T.; Wong, See-Chang; Wong, Toh-Mee; Blair, Patrick J.; Gray, Gregory C.

doi:10.1186/s40794-020-00114-2

Table 3 Results of successfully sequenced specimens positive by panspecies adenovirus [15] assay

From: Panspecies molecular assays detect viral pathogens missed by real-time PCR/reverse-transcriptase PCR among pneumonia patients, Sarawak, Malaysia

	Patient age	Hospital	Sequencing Result	Identity Score From BLAST	Accession Number from BLAST	rPCR Assay	AdV-Hexon Assay
1.	6 months	Kapit	Human mastadenovirus C	98%	MH121117.1	Neg	Neg
2.	65 years	Kapit	Human mastadenovirus E	99%	KY996453.1	Pos	Pos
3.	1 month	Kapit	Human mastadenovirus C	98%	MH121117.1	Neg	Neg
4.	66 years	Sibu	Human mastadenovirus C	97%	MH121097.1	Neg	Neg
5.	1 year	Sibu	Human adenovirus C	95%	MF358574.1	Pos	N/A^a
6.	10 months	Kapit	Human adenovirus 41	98%	KX868523.2	Neg	N/A^a
7.	6 months	Sibu	Human adenovirus C	98%	MF358562.1	Pos	N/A^a
8.	4 months	Kapit	human adenovirus 41	98%	KX868523.2	Neg	N/A^a
9.	1 year	Kapit	Human adenovirus 5	98%	MF358601.1	Pos	Pos
10.	35 years	Sibu	Human adenovirus 4	99%	AP014851.1	Pos	Pos
11.	3 years	Sibu	Human adenovirus B	99%	KF268212.1	Pos	Neg
12.	2 years	Sibu	Human adenovirus 7	99%	MG923582.1	Pos	Neg
13.	2 years	Sibu	Human adenovirus 7	98%	MG923582.1	Pos	Neg
14.	9 months	Sibu	Human adenovirus 7	98%	MG923582.1	Pos	Neg
15.	6 months	Kapit	Human adenovirus 7	97%	MG923582.1	Pos	Pos
16.	2 years	Sibu	Human adenovirus 7	99%	MG923582.1	Pos	Neg
17.	1 month	Sibu	Human adenovirus 5	99%	MF358604.1	Pos	Neg
18.	8 months	Sibu	Human adenovirus 7	98%	MG923582.1	Pos	Pos
19.	8 months	Sibu	Human adenovirus 7	98%	MG923582.1	Pos	Pos
20.	7 months	Sibu	Human adenovirus 7	98%	MG923582.1	Pos	Pos
21.	1 year	Sibu	Human adenovirus 7	97%	MG923582.1	Pos	Pos
22.	8 months	Sibu	Desmondus rotundus adenovirus 1	100%	KX774303.1	Pos	Pos
23.	1 month	Sibu	Desmondus rotundus adenovirus 1	100%	KX774303.1	Pos	Pos
24.	6 months	Sibu	Human adenovirus 7	96%	MG923582.1	Pos	Pos
25.	3 months	Sibu	Human adenovirus 7	93%	MG923582.1	Pos	Pos
26.	4 months	Sibu	Human adenovirus 7	98%	MG923582.1	Pos	Pos
27.	2 years	Sibu	Human adenovirus 7	98%	MG923582.1	Pos	Pos
28.	2 months	Sibu	Human adenovirus 7	97%	MG923582.1	Pos	Pos
29.	54 years	Sibu	Human adenovirus 5	96%	MF358604.1	Pos	Neg
30.	78 years	Sibu	Human adenovirus 5	98%	MF358604.1	Pos	Neg

^aSpecimens at Duke-NUS not run by conventional AdV-Hexon assay

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