Table 1 Primer and probe sequences and gene target region for all panspecies assays. All assays were adapted from the cited references at Duke University
Virus Assay | Function | Sequence | Target Gene |
|---|---|---|---|
Pan adenovirus [15] | |||
Nested PCR 1 | Forward primer (polFouter) | 5′-TNMGNGGNGGNMGNTGYTAYCC-3′ | Polymerase |
Reverse primer (polRouter) | 5′-GTDGCRAANSHNCCRTABARNGMRTT-3′ | ||
Nested PCR 2 | Forward primer (polFinner) | 5′-GTNTWYGAYATHTGYGGHATGTAYGC-3′ | |
Reverse primer (polRinner) | 5′-CCANCCBCDRTTRTGNARNGTRA-3′ | ||
Pan coronavirus [16] | Forward primer | 5′-GGTTGGGACTATCCTAAGTGTGA-3’ | Polymerase |
Reverse primer | 5′-CCATCATCAGATAGAATCATCATA-3’ | ||
Pan enterovirus [17] | AN32 primer | 5′ GTY TGC CA 3′ | Capsid protein VP1 |
AN33 primer | 5′ GAY TGC CA 3′ | ||
AN34 primer | 5′ CCR TCR TA 3′ | ||
AN35 primer | 5′ RCT YTG CCA 3′ | ||