Immunization of mice with soluble lysate of interferon gamma expressing Plasmodium berghei ANKA induces high IFN-γ production

Table 2 Protection of BALB/c mice immunized with mIFN-γ against wild type PbA challenge infection

Group	Pre-Patency Period (days)^a	Positive mice/Challenge (% Protected)	Mean Patent Parasitemia (%)
Group 1	7	2/4^b (50%)	0.18
Group 2^c	6	2/4^b (50%)	0.46
Group 3	3	2/5 (60%)	0.5
Group 4	3	2/4^b (50%)	2.44
Naive Control	3	2/5 (50%)	1.01

Group 1: Primed and boosted with TPbA lysate (75 pg/ml IFN-γ), Group 2: Primed with live TPbA, boosted with TPbA lysate (75 pg/ml IFN-γ); Group 3: Plain RBC control; Group 4: WT PbA control
^aThe period of detecting parasites in blood after challenge infection
^bOne mouse died unexpectedly before challenge infection. Death not due to immunization
^cMice were primed with transfected PbA and boosted twice with 35 pg/ml mIFN-γ containing soluble lysate

ISSN: 2055-0936